Fragmentation of the tryptophan cluster [Trp9–2H]2− induced by different activation methods
Identifieur interne : 007283 ( Main/Exploration ); précédent : 007282; suivant : 007284Fragmentation of the tryptophan cluster [Trp9–2H]2− induced by different activation methods
Auteurs : Linda Feketeová [Australie] ; George N. Khairallah [Australie] ; Claire Brunet [France] ; Jérôme Lemoine [France] ; Rodolphe Antoine [France] ; Philippe Dugourd [France, Australie] ; Richard A. J. O'Hair [Australie]Source :
- Rapid Communications in Mass Spectrometry [ 0951-4198 ] ; 2010-11-30.
Descripteurs français
- Wicri :
- topic : Droit d'auteur.
English descriptors
- KwdEn :
- Amino, Amino acid, Amino acid clusters, Amino acids, Analyzer, Anion, Anionic clusters, Appearance size, Charge separation, Charge state, Charge states, Chem, Cluster, Cluster anions, Cluster ions, Commun, Concomitant mass shifts, Copyright, Coulomb explosion, Deprotonated sites, Dianionic cluster, Dugourd, Electron attachment, Electron energies, Electron energy, Electrospray ionization, Emitter cathode, Fragmentation reactions, High power, High resolution, Ion, Isolation process, John wiley sons, Laser, Laser beam, Laser irradiation, Laser power, Linear quadrupole, Mass analysis, Mass analyzer, Mass spectrom, Mass spectrometer, Mass spectrometry, Mass spectrum, Mechanical shutter, Neutral tryptophan, Neutral tryptophan loss, Power dependence, Precursor, Product ions, Rapid commun, Relative abundances, Several photons, Source conditions, Spectrom, Spectrometer, Stability limit, Supplementary figs, Thermo fisher, Trap mass spectrometer, Trap spectra, Tryptophan, Tryptophan cluster, Tryptophan cluster anions, Tryptophan clusters, Tryptophan solution, Uvpd, Uvpd experiments, Wide window.
- Teeft :
- Amino, Amino acid, Amino acid clusters, Amino acids, Analyzer, Anion, Anionic clusters, Appearance size, Charge separation, Charge state, Charge states, Chem, Cluster, Cluster anions, Cluster ions, Commun, Concomitant mass shifts, Copyright, Coulomb explosion, Deprotonated sites, Dianionic cluster, Dugourd, Electron attachment, Electron energies, Electron energy, Electrospray ionization, Emitter cathode, Fragmentation reactions, High power, High resolution, Ion, Isolation process, John wiley sons, Laser, Laser beam, Laser irradiation, Laser power, Linear quadrupole, Mass analysis, Mass analyzer, Mass spectrom, Mass spectrometer, Mass spectrometry, Mass spectrum, Mechanical shutter, Neutral tryptophan, Neutral tryptophan loss, Power dependence, Precursor, Product ions, Rapid commun, Relative abundances, Several photons, Source conditions, Spectrom, Spectrometer, Stability limit, Supplementary figs, Thermo fisher, Trap mass spectrometer, Trap spectra, Tryptophan, Tryptophan cluster, Tryptophan cluster anions, Tryptophan clusters, Tryptophan solution, Uvpd, Uvpd experiments, Wide window.
Abstract
Electrospray ionization (ESI) of tryptophan gives rise to multiply charged, non‐covalent tryptophan cluster anions, [Trpn–xH]x−, in a linear ion trap mass spectrometer, as confirmed by high‐resolution experiments performed on a Fourier transform ion cyclotron resonance (FT‐ICR) mass spectrometer. The smallest multiply charged clusters that can be formed in the linear ion trap as a function of charge state are: x = 2, n = 7; x = 3, n = 16; x = 4, n = 31. The fragmentation of the dianionic cluster [Trp9–2H]2− was examined via low‐energy collision‐induced dissociation (CID), ultraviolet photodissociation (UVPD) at 266 nm and electron‐induced dissociation (EID) at electron energies ranging from >0 to 30 eV. CID proceeds mostly via charge separation and evaporation of neutral tryptophan. The smallest doubly charged cluster that can be formed via evaporation of neutral tryptophans is [Trp7–2H]2−, consistent with the observation of this cluster in the ESI mass spectrum. UVPD gives singly charged tryptophan clusters ranging from n = 2 to n = 9. The latter ion arises from ejection of an electron to give the radical anion cluster, [Trp9–2H]−.. The types of gas‐phase EID reactions observed are dependent on the energy of the electrons. Loss of neutral tryptophan is an important channel at lower energies, with the smallest doubly charged ion, [Trp7–2H]2−, being observed at 19.8 eV. Coulomb explosion starts to occur at 19.8 eV to form the singly charged cluster ions [Trpx–H]− (x = 1–8) via highly asymmetric fission. At 21.8 eV a small amount of [Trp2–H–NH3]− is observed. Thus CID, UVPD and EID are complementary techniques for the study of the fragmentation reactions of cluster ions. Copyright © 2010 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/rcm.4763
Affiliations:
- Australie, France
- Auvergne-Rhône-Alpes, Rhône-Alpes, Victoria (État)
- Lyon, Melbourne
- Université de Melbourne
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002C52
- to stream Istex, to step Curation: 002C52
- to stream Istex, to step Checkpoint: 000C00
- to stream Main, to step Merge: 007819
- to stream Main, to step Curation: 007283
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fragmentation of the tryptophan cluster [Trp9–2H]2− induced by different activation methods</title><author><name sortKey="Feketeova, Linda" sort="Feketeova, Linda" uniqKey="Feketeova L" first="Linda" last="Feketeová">Linda Feketeová</name></author><author><name sortKey="Khairallah, George N" sort="Khairallah, George N" uniqKey="Khairallah G" first="George N." last="Khairallah">George N. Khairallah</name></author><author><name sortKey="Brunet, Claire" sort="Brunet, Claire" uniqKey="Brunet C" first="Claire" last="Brunet">Claire Brunet</name></author><author><name sortKey="Lemoine, Jerome" sort="Lemoine, Jerome" uniqKey="Lemoine J" first="Jérôme" last="Lemoine">Jérôme Lemoine</name></author><author><name sortKey="Antoine, Rodolphe" sort="Antoine, Rodolphe" uniqKey="Antoine R" first="Rodolphe" last="Antoine">Rodolphe Antoine</name></author><author><name sortKey="Dugourd, Philippe" sort="Dugourd, Philippe" uniqKey="Dugourd P" first="Philippe" last="Dugourd">Philippe Dugourd</name></author><author><name sortKey="O Hair, Richard A J" sort="O Hair, Richard A J" uniqKey="O Hair R" first="Richard A. J." last="O'Hair">Richard A. J. O'Hair</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:EBF3FCCCE913D30955A2AC1AED2EC3AFC91A3600</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1002/rcm.4763</idno><idno type="url">https://api.istex.fr/document/EBF3FCCCE913D30955A2AC1AED2EC3AFC91A3600/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">002C52</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002C52</idno><idno type="wicri:Area/Istex/Curation">002C52</idno><idno type="wicri:Area/Istex/Checkpoint">000C00</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000C00</idno><idno type="wicri:doubleKey">0951-4198:2010:Feketeova L:fragmentation:of:the</idno><idno type="wicri:Area/Main/Merge">007819</idno><idno type="wicri:Area/Main/Curation">007283</idno><idno type="wicri:Area/Main/Exploration">007283</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Fragmentation of the tryptophan cluster [Trp<hi rend="subscript">9</hi>–2H]<hi rend="superscript">2−</hi> induced by different activation methods<ref type="note" target="#fn1"></ref></title><author><name sortKey="Feketeova, Linda" sort="Feketeova, Linda" uniqKey="Feketeova L" first="Linda" last="Feketeová">Linda Feketeová</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry, University of Melbourne, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>Bio21 Institute of Molecular Science and Biotechnology, the University of Melbourne, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Khairallah, George N" sort="Khairallah, George N" uniqKey="Khairallah G" first="George N." last="Khairallah">George N. Khairallah</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry, University of Melbourne, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>Bio21 Institute of Molecular Science and Biotechnology, the University of Melbourne, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Brunet, Claire" sort="Brunet, Claire" uniqKey="Brunet C" first="Claire" last="Brunet">Claire Brunet</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, CNRS et Université Lyon 1 (LASIM UMR 5579 et LSA UMR 5180), Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Lemoine, Jerome" sort="Lemoine, Jerome" uniqKey="Lemoine J" first="Jérôme" last="Lemoine">Jérôme Lemoine</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, CNRS et Université Lyon 1 (LASIM UMR 5579 et LSA UMR 5180), Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Antoine, Rodolphe" sort="Antoine, Rodolphe" uniqKey="Antoine R" first="Rodolphe" last="Antoine">Rodolphe Antoine</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, CNRS et Université Lyon 1 (LASIM UMR 5579 et LSA UMR 5180), Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation></author><author><name sortKey="Dugourd, Philippe" sort="Dugourd, Philippe" uniqKey="Dugourd P" first="Philippe" last="Dugourd">Philippe Dugourd</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Université de Lyon, CNRS et Université Lyon 1 (LASIM UMR 5579 et LSA UMR 5180), Lyon</wicri:regionArea><placeName><region type="region">Auvergne-Rhône-Alpes</region><region type="old region">Rhône-Alpes</region><settlement type="city">Lyon</settlement></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Philippe Dugourd, Université de Lyon, CNRS et Université de Lyon 1 (LASIM UMR 5579 et LSA UMR 5180), Lyon, F‐69622 France.Richard A.J. O'Hair, School of Chemistry, University of Melbourne, Victoria 3010</wicri:regionArea><wicri:noRegion>Victoria 3010</wicri:noRegion></affiliation></author><author><name sortKey="O Hair, Richard A J" sort="O Hair, Richard A J" uniqKey="O Hair R" first="Richard A. J." last="O'Hair">Richard A. J. O'Hair</name><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry, University of Melbourne, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Australie</country><wicri:regionArea>Bio21 Institute of Molecular Science and Biotechnology, the University of Melbourne, Victoria 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Australie</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Philippe Dugourd, Université de Lyon, CNRS et Université de Lyon 1 (LASIM UMR 5579 et LSA UMR 5180), Lyon, F‐69622 France.Richard A.J. O'Hair, School of Chemistry, University of Melbourne, Victoria 3010</wicri:regionArea><wicri:noRegion>Victoria 3010</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Rapid Communications in Mass Spectrometry</title><title level="j" type="alt">RAPID COMMUNICATIONS IN MASS SPECTROMETRY</title><idno type="ISSN">0951-4198</idno><idno type="eISSN">1097-0231</idno><imprint><biblScope unit="vol">24</biblScope><biblScope unit="issue">22</biblScope><biblScope unit="page" from="3255">3255</biblScope><biblScope unit="page" to="3260">3260</biblScope><biblScope unit="page-count">4</biblScope><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2010-11-30">2010-11-30</date></imprint><idno type="ISSN">0951-4198</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0951-4198</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino</term><term>Amino acid</term><term>Amino acid clusters</term><term>Amino acids</term><term>Analyzer</term><term>Anion</term><term>Anionic clusters</term><term>Appearance size</term><term>Charge separation</term><term>Charge state</term><term>Charge states</term><term>Chem</term><term>Cluster</term><term>Cluster anions</term><term>Cluster ions</term><term>Commun</term><term>Concomitant mass shifts</term><term>Copyright</term><term>Coulomb explosion</term><term>Deprotonated sites</term><term>Dianionic cluster</term><term>Dugourd</term><term>Electron attachment</term><term>Electron energies</term><term>Electron energy</term><term>Electrospray ionization</term><term>Emitter cathode</term><term>Fragmentation reactions</term><term>High power</term><term>High resolution</term><term>Ion</term><term>Isolation process</term><term>John wiley sons</term><term>Laser</term><term>Laser beam</term><term>Laser irradiation</term><term>Laser power</term><term>Linear quadrupole</term><term>Mass analysis</term><term>Mass analyzer</term><term>Mass spectrom</term><term>Mass spectrometer</term><term>Mass spectrometry</term><term>Mass spectrum</term><term>Mechanical shutter</term><term>Neutral tryptophan</term><term>Neutral tryptophan loss</term><term>Power dependence</term><term>Precursor</term><term>Product ions</term><term>Rapid commun</term><term>Relative abundances</term><term>Several photons</term><term>Source conditions</term><term>Spectrom</term><term>Spectrometer</term><term>Stability limit</term><term>Supplementary figs</term><term>Thermo fisher</term><term>Trap mass spectrometer</term><term>Trap spectra</term><term>Tryptophan</term><term>Tryptophan cluster</term><term>Tryptophan cluster anions</term><term>Tryptophan clusters</term><term>Tryptophan solution</term><term>Uvpd</term><term>Uvpd experiments</term><term>Wide window</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid</term><term>Amino acid clusters</term><term>Amino acids</term><term>Analyzer</term><term>Anion</term><term>Anionic clusters</term><term>Appearance size</term><term>Charge separation</term><term>Charge state</term><term>Charge states</term><term>Chem</term><term>Cluster</term><term>Cluster anions</term><term>Cluster ions</term><term>Commun</term><term>Concomitant mass shifts</term><term>Copyright</term><term>Coulomb explosion</term><term>Deprotonated sites</term><term>Dianionic cluster</term><term>Dugourd</term><term>Electron attachment</term><term>Electron energies</term><term>Electron energy</term><term>Electrospray ionization</term><term>Emitter cathode</term><term>Fragmentation reactions</term><term>High power</term><term>High resolution</term><term>Ion</term><term>Isolation process</term><term>John wiley sons</term><term>Laser</term><term>Laser beam</term><term>Laser irradiation</term><term>Laser power</term><term>Linear quadrupole</term><term>Mass analysis</term><term>Mass analyzer</term><term>Mass spectrom</term><term>Mass spectrometer</term><term>Mass spectrometry</term><term>Mass spectrum</term><term>Mechanical shutter</term><term>Neutral tryptophan</term><term>Neutral tryptophan loss</term><term>Power dependence</term><term>Precursor</term><term>Product ions</term><term>Rapid commun</term><term>Relative abundances</term><term>Several photons</term><term>Source conditions</term><term>Spectrom</term><term>Spectrometer</term><term>Stability limit</term><term>Supplementary figs</term><term>Thermo fisher</term><term>Trap mass spectrometer</term><term>Trap spectra</term><term>Tryptophan</term><term>Tryptophan cluster</term><term>Tryptophan cluster anions</term><term>Tryptophan clusters</term><term>Tryptophan solution</term><term>Uvpd</term><term>Uvpd experiments</term><term>Wide window</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Droit d'auteur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Electrospray ionization (ESI) of tryptophan gives rise to multiply charged, non‐covalent tryptophan cluster anions, [Trpn–xH]x−, in a linear ion trap mass spectrometer, as confirmed by high‐resolution experiments performed on a Fourier transform ion cyclotron resonance (FT‐ICR) mass spectrometer. The smallest multiply charged clusters that can be formed in the linear ion trap as a function of charge state are: x = 2, n = 7; x = 3, n = 16; x = 4, n = 31. The fragmentation of the dianionic cluster [Trp9–2H]2− was examined via low‐energy collision‐induced dissociation (CID), ultraviolet photodissociation (UVPD) at 266 nm and electron‐induced dissociation (EID) at electron energies ranging from >0 to 30 eV. CID proceeds mostly via charge separation and evaporation of neutral tryptophan. The smallest doubly charged cluster that can be formed via evaporation of neutral tryptophans is [Trp7–2H]2−, consistent with the observation of this cluster in the ESI mass spectrum. UVPD gives singly charged tryptophan clusters ranging from n = 2 to n = 9. The latter ion arises from ejection of an electron to give the radical anion cluster, [Trp9–2H]−.. The types of gas‐phase EID reactions observed are dependent on the energy of the electrons. Loss of neutral tryptophan is an important channel at lower energies, with the smallest doubly charged ion, [Trp7–2H]2−, being observed at 19.8 eV. Coulomb explosion starts to occur at 19.8 eV to form the singly charged cluster ions [Trpx–H]− (x = 1–8) via highly asymmetric fission. At 21.8 eV a small amount of [Trp2–H–NH3]− is observed. Thus CID, UVPD and EID are complementary techniques for the study of the fragmentation reactions of cluster ions. Copyright © 2010 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li><li>Victoria (État)</li></region><settlement><li>Lyon</li><li>Melbourne</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Feketeova, Linda" sort="Feketeova, Linda" uniqKey="Feketeova L" first="Linda" last="Feketeová">Linda Feketeová</name></region><name sortKey="Dugourd, Philippe" sort="Dugourd, Philippe" uniqKey="Dugourd P" first="Philippe" last="Dugourd">Philippe Dugourd</name><name sortKey="Feketeova, Linda" sort="Feketeova, Linda" uniqKey="Feketeova L" first="Linda" last="Feketeová">Linda Feketeová</name><name sortKey="Khairallah, George N" sort="Khairallah, George N" uniqKey="Khairallah G" first="George N." last="Khairallah">George N. Khairallah</name><name sortKey="Khairallah, George N" sort="Khairallah, George N" uniqKey="Khairallah G" first="George N." last="Khairallah">George N. Khairallah</name><name sortKey="O Hair, Richard A J" sort="O Hair, Richard A J" uniqKey="O Hair R" first="Richard A. J." last="O'Hair">Richard A. J. O'Hair</name><name sortKey="O Hair, Richard A J" sort="O Hair, Richard A J" uniqKey="O Hair R" first="Richard A. J." last="O'Hair">Richard A. J. O'Hair</name><name sortKey="O Hair, Richard A J" sort="O Hair, Richard A J" uniqKey="O Hair R" first="Richard A. J." last="O'Hair">Richard A. J. O'Hair</name><name sortKey="O Hair, Richard A J" sort="O Hair, Richard A J" uniqKey="O Hair R" first="Richard A. J." last="O'Hair">Richard A. J. O'Hair</name></country><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Brunet, Claire" sort="Brunet, Claire" uniqKey="Brunet C" first="Claire" last="Brunet">Claire Brunet</name></region><name sortKey="Antoine, Rodolphe" sort="Antoine, Rodolphe" uniqKey="Antoine R" first="Rodolphe" last="Antoine">Rodolphe Antoine</name><name sortKey="Dugourd, Philippe" sort="Dugourd, Philippe" uniqKey="Dugourd P" first="Philippe" last="Dugourd">Philippe Dugourd</name><name sortKey="Dugourd, Philippe" sort="Dugourd, Philippe" uniqKey="Dugourd P" first="Philippe" last="Dugourd">Philippe Dugourd</name><name sortKey="Lemoine, Jerome" sort="Lemoine, Jerome" uniqKey="Lemoine J" first="Jérôme" last="Lemoine">Jérôme Lemoine</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 007283 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 007283 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:EBF3FCCCE913D30955A2AC1AED2EC3AFC91A3600
|texte= Fragmentation of the tryptophan cluster [Trp9–2H]2− induced by different activation methods
}}
| This area was generated with Dilib version V0.6.33. |  |